Currently, piscirickettsiosis is the primary cause for bacteria-related fish mortalities, translating into significant economic losses in the Chilean salmon industry (Sernapesca 2017).

Antibiotics treatment, mainly florfenicol, can lead to drug resistance in this pathogen (Sandoval et al. Piscirickettsia salmonis, the etiological agent of piscirickettsiosis, is a facultative intracellular Gram-negative bacterium that was initially isolated from a diseased Coho salmon ( Oncorhynchus kisutch) in southern Chile in 1989 (Bravo and Campos 1989 Branson and Nieto Diaz-Munoz 1991). Piscirickettsia salmonis, SRS, piscirickettsiosis, type IV pilus, pili INTRODUCTION These results strongly suggest the presence of a T4P-like structure in P. salmonis LF-89 T proteome, and TEM showed pili-like filamentous structures on the P. The PilA amino acid sequence analysis showed a conserved N-terminal domain and sequence motifs critical for T4P biosynthesis. At 16 hpi, pilB and pilD were strongly upregulated. Expression of the pilA gene was upregulated at 4 h post-infection (hpi), while pilQ was upregulated 4 days post-infection.

salmonis type strain LF-89 T, evaluate respective transcript expressions, and analyze the main putative T4P proteins using bioinformatics and proteomic approaches. The aims of this investigation were to identify T4P components in the P. However, no studies have determined if P. T4P contains a variable number of components, as predicted in P. The type IV pili (T4P) play an important role in adherence to host cell surfaces and bacterial pathogenicity. Piscirickettsia salmonis is an intracellular γ-proteobacteria and the etiological agent of piscirickettsiosis, which causes massive economic losses in the Chilean salmon industry.

Amplifx in silico pcr triangle